Dose-dependent cytotoxicity effect of Aspirin on MCF7 cell line proliferation

Abstract

NSAID). Because aspirin nonselectively blocks COX-1 and COX-2 via irreversible acetylation. COX-2 regulates many functions such as augmentation of apoptosis, inhibition of angiogenesis, and it has anticancer effect. The aim of the present study was to detect Dose dependent cytotoxicity effect of Aspirin on MCF7 cell line proliferation and activity.  Aspirin concentrations (1000, 500, 250, 125, 62.5, 31.2µg/ml). aspirin, was tested for cytotoxicity against the breast cancer cell line MCF7 using the MTT assay. The results showed that aspirin has cytotoxicity against MCF7 cells. the IC50 of the aspirin dose dependent treated range (from 243.3 to 888.6 µg/ml was (465 µg/ml). reduction in the growth, viability, proliferation, and change in morphology of cancer cells and apoptosis, all this effect increased with the increasing concentration of aspirin. Growth inhibition (GI) at highest concentration (1000µg/ml).is 35.5 %.and in lowest concentration (31.2µg/ml) is 4.8 %. In conclusion, aspirin has anticancer cytotoxicity effect.