Determination Effect of Colchicine on the Fertility of Adult Male Rats

Abstract

This study was carried out to  preparation of the chemical colchicine at a dose of (2.5 mg/kg body weight) , which, in the form of a powder in a sealed. And determines its effect on male fertility by evaluate the physiological bioassay of both sexual hormone and sperm parameters .

     This study aimed at the determining the side effects of Colchicine and effectiveness on the sperm and the fertility of adult male rats. The experiment was carried out at Tikrit University's College of Veterinary Medicine in the animal house. Hence, 20 adult male rats  and their weight was 300 gram were used for physiological bioassay challenge of the colchicine . The animals were split up into Two groups, with ten animals in each group for a duration of thirty days. First, the distilled water was administered as the control group, whereas oral colchicine (2.5 mg/kg body weight) was administered once a day to the second group.

      The results of the study indicated that the group receiving colchicine alone had significantly lower levels of FSH (P ≤ 0.05) (0.1280 ± 0.0316) when compared with control group (0.2390 ± 0.0119). Additionally, the group treated with colchicine alone revealed a significant decrease (P≤0.05) in the level of testosterone hormone (2.280 ± 1.080) compared with control group (4.363 ± 1.337) and a significant decrease (P ≤ 0.05) in the level of LH (0.1120 ± 0.0103) compared with control group (0.2120 ± 0.0193).

     The study found that the group receiving colchicine alone had significantly (P ≤ 0.05)  fewer sperm (3.500 ± 1.080) compared with control group (6.300 ± 1.337). Furthermore demonstrated a significant decrease (P ≤ 0.05) in sperm viability in the colchicine-only group (71.500 ± 1.958) compared with control group (89.900 ± 1.792) . Additionally, significant decrease (P ≤ 0.05) in sperm motility in the colchicine-only group demonstrated (43.330 ± 1.958) compared with control group(82.570 ± 1.792) and a significant increase (P ≤ 0.05) in the sperm abnormality demonstrated in the colchicine-only group (46.400 ± 1.958 ) compared with control group (18.600 ± 1.792) .

        Colchicine treatment had an impact on the fertility of adult male rats, resulting in decreased testosterone, FSH and LH levels as well as decreased sperm motility, count, and viability and increased sperm abnormalities.