Histological and biochemical effects of diclofenac sodium in adult mice

Abstract

This study was designed to investigate the adverse effects of a daily single injection of Diclofenac sodium (DS) at two doses on some histological and biochemical parameters in adult mice. The study conducted on 15 of adult mice. Animals were divided into three groups, consisted of 5 animals. Group (I) served as control group and received distilled water only, while group (II) has been set as treated injection with diclofenac sodium 5mg /kg bw for 21 days, group (III) has been treated with diclofenac sodium 10 mg/ Kg bw for 21 days. The following parameters were studied in experimental groups included the histologically effect on kidney, and liver, biochemical tests to evaluated the activity of the enzymes aspartate aminotransferase (AST) ,alanine aminotransferase (ALT) and Alkaline phosphatase (ALP) . The results showed that Ds at dose of 5 mg / kg and 10 mg /Kg induce a significant increase in the level of the activity of the enzymes , (AST ,ALT and ALP)  at p value P<0.05. DS showed significant alteration in kidney and liver tissues compared to control group, for instance ,histological studies in mice treated with DS (5mg /kg )showed massive aggregation of lymphocytic and other white blood cell (WBC)in their kidneys ,also hepatic tissues treated with DS(5mg/kg )were clearly affected and showed hypertrophy of parenchymal tissues and sinusoids were narrowing with kupffer cells. Similarly ,sever and marked damage were found in mice kidney, and liver tissues after treatment with DS(10mg/kg). Overall, these results revealed the toxic effect of DS at 5 and 10 mg /kg on mice kidney ,and liver