Effect of Moringa oleifera Extract on blood Levels of Lipocalin-2 and Omentin Parameters in High Fat Diet-induced Obesity in Rats.

Abstract

The present study was carried out to investigate the efficacy of Moringa Oleifera (MO). Forty Wistar rats with body weight (150-200gm) and were randomly divided into four (4) groups and treated for 12 weeks: group I- rats treated with normal diet (Control), group II: Obese group received HFD for 12 weeks, group III `- Obese group rats treated with HFD for 12 weeks then received with MO extract orally in a dose of 600 mg kg^-1 b.wt, and group IV: Obese group treated with HFD for 12 weeks then received with simvastatin at a daily dose for 12 weeks of 5 mg kg^-1 b.wt .Blood samples were collected for studying biochemical changes (Lipocalin-2 and omentin ) associated with the use of MO extract on HFD -induced diabetic rats. The present study  indicated that  administration of high fat diet in rats led to an significant increase in body mass index (BMI) in t that is in group II , compared to obese group. On the other side, in group III &group IV treatment with MOE and Simvastatin resulted in significant decrease in BMI compared to HFD  group. There was significant increase in the levels of serum Lipocalin-2 (172.6 ± 1.73ng/μl)whereas significant decrease omentin-1 (20 ± 0.57 pg/ml)  in the HFD animals when compared to normal fed rats(63.94 ± 2.19 ng/μl), and(38.15 ± 1.071 pg/ml).On the other hand the levels of  Lipocalin-2 decreased  and omentin-1 (20 ± 0.57 pg/ml  increased in the obese  group following MO(118.5 ± 1.21 ng/μl), (30.62 ± 0.94pg/ml) and Simvastatin treatment(116 ± 1.83ng/μl), (31.70 ± 0.871 pg/ml) respectively. The results indicate that MO  has anti‑obesity effects in an experimental mouse model of high‑fat‑diet‑induced obesity.