Histopathological evaluation of Tilduronate on healing of femoral bone in dogs

Abstract

The study was designed to evaluate the clinical and histopathological effects produced by the systemic administration of Tildronate (Bisphosphenates compound), on the healing of experimentally induced femoral bone fracture in twelve adult dogs, at which the fractures were fixed by Steinman intramedullary pins. The dogs were divided randomly into two groups, six for each. Following induction of femoral bone fracture and intramedullar fixation, the dogs in the treated group were treated with tiludronic acid at 2 mg / kg body weight, subcutaneously, twice weekly, for eight consecutive weeks. In control group the fracture was fixed by steinmam's intramedullary pin and was left to repair spontaneously without further medical treatment . All dogs were followed for two months by routine clinical inspection and the animals of other group were scarified at; 21, 45, and 60 days, respectively, to collect specimens from the fracture site to prior histopathological examination. The results revealed that the potential active effect of Tiludronate on the course of fracture healing was to minimize the activity and life span of osteoclast cells at the fracture site, contributing to a pause in bone remodeling. The influence of this effect was demonstrated by the delay in the time taken by the bone to regain its normal shape in the treated community, compared with the time taken by the control group.