Isolation and diagnosis of Pseudomonas aeruginosa using traditional and Virtek-2 techniques

Abstract

This study has been conducted in order to isolate and identify Pseudomonas aeruginosa from human urinary tract infections using traditional methods and the VITEK-2 system. Where , 120 patients with urinary tract infections participated. 20 samples were taken using a catheter and 100 urine samples were taken without a catheter. The samples were incubated at 37°C for 24 hours using brain heart infusion broth, then Pseudo agar, Cetrimide agar, and MacConkey agar. The bacterial isolates were purified and diagnosed by conventional biochemical tests. Then the pure cultures underwent examination by the Vitek-2 system.  Bacterial isolates on MacConkey agar showed small, pale colonies, while Cetrimide agar showed bacterial isolates as smooth, mucous-shaped colonies. The use of the Vitek-2 system confirmed that all suspected colonies were isolates of Pseudomonas aeruginosa. The results of the current study revealed the isolation of sixty-eight (68 isolates) Pseudomonas aeruginosa isolates. In Conclusion,  Vitek-2 technology can quickly and accurately identify clinically important germs, including Pseudomonas aeruginosa.