Hormonal and Histological effect of Nitric oxide on reproductive system of female mice

Abstract

The present study aimed to study the pharmacological effect of NO donor Sodium Nitroprusside on female reproductive system in mice through evaluate the levels of hormones  (FSH , LH , Estrogen  and progesterone) in blood serum and histology changes in ovaries tissue. The experiment was counducted on the Thirty female mice in good health, weighting ranged between )25-34) gm,  were divided into six groups (five animals for each group) and treated by sodium nitroprusside (Intraperitoniolly) located in the animal house of veterinary medicine college/ Tikrit university were used in the current study. after 21 days of treatment by  (SNP). The results of current study showed a significant increase (P< 0.05) in all treated groups, compared with control group, while it showed significant decrease (P<0.05) in progesterone hormone  gradually with the increase in  SPN dose in all groups compared with the control group. The histological evaluation results of average numbers of follicles per ovary in the treated groups, for determining the primordial follicles ,primary follicles and secondary follicles number showed a significant increased (P<0.05) in all treated groups when compared with control group , The G6 gradually  decreased (P< 0.05) compared with other treated groups, The tertiary  follicles number in all groups except the G2 showed a significant decrease (P< 0.05) compared with the  control group, The results of ovaries histological changes of ovary showed cytoplasmic degeneration and vacuolation of germinal epithelium. also, certain Follicular cells were degenerated and surrounded by WBCs infiltration, Most of the primordial cells degenerated with the  presence of inflammatory exudate between those cells and degeneration of oocyte of the tertiary follicle  surrounded with many degenerated Follicular cells and infiltration of WBCs in the cortical tissue between the primordial follicle, the blood vessels of medulla were containing congested, hemolysis blood with increased of dosage, The study concluded the positive effect on the reproductive system in the female mice, but the effects appeared in the high doses of treatment.