Evaluation the effects of Urtica dioica and Zingiber officinale extracts on Cryptosporidium hominis cultured on free-cell media by using RAPD technique

Abstract

The aim of this study was to explore the potential activities of the alcoholic and aqueous extracts of Urtica dioica leaves and Zingiber officinale rhizome on Cryptosporidium hominis that were grown in vivo,and evaluate the anticryptosporidial effect(s) at the molecular level by measuring the DNA concentration and using random amplification of polymorphic DNA (RAPD) analysis of the genomic DNA extracted from the control (untreated) group and plant extracts-treated groups. The results demonstrate that the alcoholic extract of U. dioica has an effect on the DNA concentration of tested parasite more than of Z. officinale .