Histomorphology and histochemical study of the spleen in postnatal puppies

Abstract

Background and aim: The spleen is an immune organs the main function have been attributed to the spleen it stores and concentrates erythrocytes and releases them when need ,it filterated the blood and remove  worn out erythrocyte from circulation .the study aims to identify the morphometries of the postnatal' spleen in dogs and to add information about the spleen of puppies to the data in clinical and academic examinations.

Materials and Methods: Nine puppies were taken after birth and dissected after euthanasia.These puppies were divided into two groups: the first group for macroscopic study and the second group for microscopic

Results: The macroscopic results revealed that the spleen in puppies is situated beneath the diaphragm on the left side. The spleen was oblong, crescent-shaped, reddish-brown in color, related to the jejunum ventrally, the liver and stomach dorsally, and the abdominal wall laterally and had two surfaces. The microscopic result in the postnatal spleen, surrounded by a thin capsule, extended the trabeculae to stroma and became thick near to hilus. The parenchyma of the spleen consists of an aggregation of lymphoid cells around the arterioles while the red pulp consists of the cord of sinuses lined by endothelium cells.

Conclusions: no demarcated structure of the spleen between the three weeks of age.